ABSTRACT
@#This study aimed to determine the in vivo effectiveness of the ethanolic extract of Piper betle L. leaves against Staphylococcus aureus-infected wounds in mice and its antimicrobial properties on clinical isolates of multiple drug-resistant bacterial pathogens. Twenty mice were divided into four groups. Wounds were created in all mice under anesthesia by excision from the dorsal skin down to the subcutaneous fat and inoculating with S. aureus. After 24 h, the wound of each mouse was treated once daily by application of the respective cream. Group I was treated with mupirocin antibacterial cream; Group II received a cream base containing no active ingredient; Groups III and IV were treated with 2.5% and 5.0% concentrations of P. betle cream, respectively. Further, an in vitro study was performed by adding undiluted, 1:50 and 1:100 dilutions of the four studied creams in normal saline containing 1.5 × 108 CFU/mL of the following bacteria: antimicrobial-susceptible S. aureus, Escherichia coli, Pseudomonas aeruginosa, methicillin-resistant S. aureus, extended-spectrum β-lactamase-producing Escherichia coli, vancomycin-resistant Enterococcus, metallo-βlactamase-producing P. aeruginosa and carbapenem-resistant Klebsiella pneumoniae. The mice in Groups III and IV had significantly faster wound contraction and significantly shorter reepithelialization time than Group II (p < 0.05), which were not significantly different from Group I (p > 0.05). P. betle creams inhibited all studied bacterial strains at full concentration and at a dilution of 1:50. The inhibitory effect was more significant than Groups I and II (p < 0.05), except on S. aureus. Specifically, S. aureus inhibition was not significantly different for Groups III and IV (p > 0.05) when compared with Group I. Cream formulations derived from P. betle ethanolic extract have great potential as antimicrobial agents for the treatment of wound infection. Further clinical tests are recommended to determine the safety and efficacy of these formulations in other mammalian species.
ABSTRACT
Children with hematological diseases usually accompanied by low autoimmune function,and repeated chemotherapy exacerbated the damage to their immune system and hematopoietic function.Those lead to high incidence of nosocomial infection,most of infection were caused by multi-drug resistant bacteria and fungi.The major infections in hematological children are the following:multi-drug resistant Escherichia coli/Klebsiella pneumonia bacteria;multi-drug resistance Pseudomonas and Acinetobacte;Methicillin-resistant coagulase negative Staphylococcus and aureus;multi-drug resistance Enterococcus faecium.This review presents updated treatment strategies from the published clinical literature and provides recommendations for clinical treatment of multi-drug resistant bacteria in children with hematonosis.
ABSTRACT
Objective: To analyze the condition of infections caused by MDROs and effect of hospital infection monitoring measures. Methods: Patients with MDROs positive from July 2009 to December 2012were prospectively studied and given Bundle monitoring measures, patients with MDROs positive from January 2006 to June 2012 were retrospectively studied, the condition of hospital acquired infections caused by MDROs and the value of hospital infection monitoring measures were analyzed. Results:(1)the total cases of MDROs infection were 1782, including 839 cases of hospital infection and 944 cases of community infection, separately accounted for 47.08% and 52.92%; the incidence of ESBLs-ECO and MRSA were highest, separately 30.04%,39.09%;(2)after monitoring measures, the incidence of MDROs in surgery department(x2=15.273, P=0.001), internal medicine department(x2=7.532, P=0.021), gynaecology(x2=11.842, P=0.008) and obstetrics department(x2=10.842, P=0.010), paediatrics department(x2=8.834, P=0.017) were lower than those of before monitoring measures;(3)the incidence of hospital acquired infections caused by MDROs were negatively correlated with effective monitoring measures and positively correlated with ommunity infection. Conclusion:Bundle monitoring measures can contribute to the control of hospital acquired infections caused by MDROs.